Banner: High Resolution Electron Microscopy at NIH  
skip navigation
Home
Lab Members
Research
Publications
Journal Covers
Photo Gallery
Join the Lab
News
 
NIH Scientists Visualize Structures of Brain Receptors
NIH News
 
Molecular Mechanism Identified for Neurotransmitter Receptor in the Brain
NCI News Note
 
Fellows Award for Research Excellence (FARE)
Awarded to Doreen Matthies and Gabriel Frank
 
PrEParing for HIV: An Epidemic Initiatives Intervention
University of California Television
 
HIV and Influenza Share a Similar Structural Blueprint
NCI News Note
 
Protein Machines at Work
NIH Director's Blog
 
Small Antibody Fragments Bind and Neutralize HIV
NCI in the Journals
 
The Living Lab: Navigating into Cells
NCI News
 
Cell Picture Show: Viruses
Cell
 
Unraveling the
structural basis of
HIV-1 neutralization

Future Microbiology
 
Structure of the pre-fusion state of the HIV Env trimer determined
IAVI Report
 
HIV Protein Strikes a Fleeting Pose
NIH Research Matters
 
How HIV Hijacks the Immune System
National Public Radio
 
NCI scientists image proteins on HIV surface
NCI News Note
 
NIH-FEI Living Lab for Structural Biology
1. NIH Press Release
2. Chemical and Engineering News
Living Lab Website
2012 Intramural AIDS Research Fellowship
Awarded to Joel Meyerson
NIH Office of Intramural Training
 
Adobe Acrobat Reader icon get Adobe Reader
Remote Data Acquisition
ASBMB Today
 
Molecular Structures of HIV Glycoproteins
Journal of Visualized Experiments
 
Cell Picture Show: HIV
Cell
 
2011 Intramural AIDS Research Fellowship
Awarded to Gabriel Frank
 

Artist’s Rendition of HIV’s Surface
July 27, 2010
IAVI Report

 
HIV Spread in 3D
Nature Research Highlights
 
Visualizing High-Efficiency HIV Transfer
NCI in the Journals
 
Students Accelerate HIV Research
CCR Connections
 
The Beauty Behind the Beasts
IAVI Report
 
A Conversation with Dr. Sriram Subramaniam
NCI Cancer Bulletin
 
HIV Molecular Entry Claw
1. NIH News
2. NIH Research Matters

 

 

   
WELCOME


From molecules to tissues: Bridging the gap with
three-dimensional electron microscopy

 


For many years, electron microscopy has been used to image cells and tissues at high resolution. This technology, invented in the early 20th century, provided breakthrough information in the virology and cell biology fields. Over the last 15 to 20 years, however, rapid advances in imaging and computation technologies have expanded the usefulness of electron microscopy into new realms. Electron microscopy is now poised to close a critical "gap" in the structural biology field.

The most commonly used methodologies in the structural biology field are X-ray crystallography and nuclear magnetic resonance spectroscopy (NMR). While each of these methods have yielded many key structures, many of the molecular complexes of greatest importance to biomedical research are either too large for NMR or are too conformationally heterogeneous for crystallization. Due to recent advances in a number of areas, electron microscopy now has the capability to determine structures of protein complexes like these to high resolution.

Our laboratory focuses on exploring the frontiers of electron microscopy, using this technology to determine three-dimensional structures of molecular complexes and whole cells at high resolution.

 
  Cell Press Picture Show gallery image
   
 

The long-term mission of the laboratory is to obtain an integrated molecular understanding of cellular architecture by combining novel technologies for 3D biological imaging with advanced methods for image segmentation and computational analysis. Principal areas of current and future focus in the laboratory are:

  • Structural analysis of protein complexes involved in signaling and metabolism

  • Enveloped virus glycoproteins: structures and applications to vaccine design

  • 3D subcellular imaging with correlative light and electron microscopy

  • Development of methods for high resolution cryo-EM

   
  This figure shows a schematic comparison on a single size scale of the relative sizes of various objects that are of interest in biology.
  Adapted from review by Subramaniam in Curr. Opin. In Microbiology (2005)
 
 
National Institutes of Health (NIH)
USA.gov: The U.S. Government's Official Web Portal
Department of Health and Human Services
United States Department of Health and Human Services
National Cancer Institute \ Center for Cancer Research \ Laboratory of Cell Biology