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Fellows Award for Research Excellence (FARE)
Awarded to Doreen Matthies and Gabriel Frank
 
PrEParing for HIV: An Epidemic Initiatives Intervention
University of California Television
 
HIV and Influenza Share a Similar Structural Blueprint
NCI News Note
 
Protein Machines at Work
NIH Director's Blog
 
Small Antibody Fragments Bind and Neutralize HIV
NCI in the Journals
 
The Living Lab: Navigating into Cells
NCI News
 
Cell Picture Show: Viruses
Cell
 
Unraveling the
structural basis of
HIV-1 neutralization

Future Microbiology
 
Structure of the pre-fusion state of the HIV Env trimer determined
IAVI Report
 
HIV Protein Strikes a Fleeting Pose
NIH Research Matters
 
How HIV Hijacks the Immune System
National Public Radio
 
NCI scientists image proteins on HIV surface
NCI News Note
 
NIH-FEI Living Lab for Structural Biology
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2012 Intramural AIDS Research Fellowship
Awarded to Joel Meyerson
NIH Office of Intramural Training
 
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Remote Data Acquisition
ASBMB Today
 
Molecular Structures of HIV Glycoproteins
Journal of Visualized Experiments
 
Cell Picture Show: HIV
Cell
 
2011 Intramural AIDS Research Fellowship
Awarded to Gabriel Frank
 

Artist’s Rendition of HIV’s Surface
July 27, 2010
IAVI Report

 
HIV Spread in 3D
Nature Research Highlights
 
Visualizing High-Efficiency HIV Transfer
NCI in the Journals
 
Students Accelerate HIV Research
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The Beauty Behind the Beasts
IAVI Report
 
A Conversation with Dr. Sriram Subramaniam
NCI Cancer Bulletin
 
HIV Molecular Entry Claw
1. NIH News
2. NIH Research Matters

 

 

   
WELCOME


From molecules to tissues: Bridging the gap with
three-dimensional electron microscopy

 


Emerging methods in three-dimensional biological electron microscopy provide powerful tools and great promise to bridge a critical gap in imaging in the biomedical size spectrum. This gap comprises a size range of considerable interest in biology and medicine that includes cellular protein machines, giant protein and nucleic acid assemblies, small subcellular organelles and small bacteria. These objects are generally too large and/or too heterogeneous to be investigated by high resolution X-ray and NMR methods; yet the level of detail afforded by conventional light and electron microscopy is often not adequate to describe their structures at resolutions high enough to be useful in understanding the chemical basis of biological function.

 
  SPECIAL FEATURE!
  Cell Press Picture Show gallery image
  Work from our laboratory is prominently featured in a recent gallery (June 2011) at Cell marking the 30th anniversary of HIV research.
To view the gallery go to: http://www.cell.com/Cell_Picture_Show-hiv
   
 

The long-term mission of our research program is to obtain an integrated molecular understanding of cellular architecture by combining novel technologies for 3D biological imaging with advanced methods for image segmentation and computational analysis. Principal areas of current and future focus in the laboratory are:

  • determination of the dynamic architecture of the bacterial chemotaxis apparatus and quantitative description of the mechanisms of signaling

  • 3D structure, mechanisms of neutralization and cellular entry of HIV

  • structure determination of dynamic molecular machines involved in energy transduction

  • the development and application of novel technologies for three-dimensional electron microscopy, especially as applicable to cancer research

We are taking an interdisciplinary approach to achieve these goals by combining high-resolution electron microscopy with a variety of complementary biological and computational methods.

   
  This figure shows a schematic comparison on a single size scale of the relative sizes of various objects that are of interest in biology.
  Adapted from review by Subramaniam in Curr. Opin. In Microbiology (2005)
   
 
 
Link to timeline: 30 years of AIDS vaccine research

See our landmark 2008 Nature publication featured in IAVI Report timeline of the 30-year history of HIV research.

Nature article: http://www.ncbi.nlm.nih.gov/pubmed/18668044

PDF of timeline: 30 years of IAVI Report AIDS vaccine research
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