The long-term mission of our research program is to obtain an integrated molecular understanding of cellular architecture by combining novel technologies for 3D biological imaging with advanced methods for image segmentation and computational analysis. Principal areas of current and future focus in the laboratory are:
- determination of the dynamic architecture of the bacterial chemotaxis apparatus
and quantitative description of the mechanisms of signaling
- 3D structure, mechanisms of neutralization and cellular entry of HIV
- structure determination of dynamic molecular machines involved in energy transduction
- the development and application of novel technologies for three-dimensional electron microscopy, especially as applicable to cancer research
We are taking an interdisciplinary approach to achieve these goals by combining high-resolution electron microscopy with a variety of complementary biological and computational methods.