High Resolution Electron Microscopy at the National Cancer Institute


	Protein Machines at Work NIH Director's Blog

	Small Antibody Fragments Bind and Neutralize HIV NCI in the Journals

	The Living Lab: Navigating into Cells NCI News

	Cell Picture Show: Viruses Cell

	Unraveling the structural basis of HIV-1 neutralization Future Microbiology

	Structure of the pre-fusion state of the HIV Env trimer determined IAVI Report

	HIV Protein Strikes a Fleeting Pose NIH Research Matters

	How HIV Hijacks the Immune System National Public Radio

	NCI scientists image proteins on HIV surface NCI News Note

	NIH-FEI Living Lab for Structural Biology 1. NIH Press Release 2. Chemical and Engineering News

Living Lab Website

	2012 Intramural AIDS Research Fellowship Awarded to Joel Meyerson NIH Office of Intramural Training

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	Remote Data Acquisition ASBMB Today

	Molecular Structures of HIV Glycoproteins Journal of Visualized Experiments

	Cell Picture Show: HIV Cell

	2011 Intramural AIDS Research Fellowship Awarded to Gabriel Frank

Artist’s Rendition of HIV’s Surface
July 27, 2010
IAVI Report


	HIV Spread in 3D Nature Research Highlights

	Visualizing High-Efficiency HIV Transfer NCI in the Journals

	Students Accelerate HIV Research CCR Connections

	The Beauty Behind the Beasts IAVI Report

	A Conversation with Dr. Sriram Subramaniam NCI Cancer Bulletin

	HIV Molecular Entry Claw 1. NIH News 2. NIH Research Matters


	PUBLICATIONS Selected Publications: 1999 - 2013

	2010-2013 \| 2005-2009 \| 1999-2004

Harris AK, Bartesaghi A, Milne JLS, and Subramaniam S. (2013) HIV-1 envelope glycoprotein trimers display open quaternary conformation when bound to the gp41 MPER-directed broadly neutralizing antibody Z13e1.
J Virol. 2013 Apr 17. [Epub ahead of print]

Merk A and Subramaniam S. (2013) HIV-1 envelope glycoprotein structure.
Curr Opin Struct Biol. 2013 Apr 18. pii: S0959-440X(13)00049-3. doi: 10.1016/j.sbi.2013.03.007. [Epub ahead of print]

Schauder DM, Kuybeda O, Zhang J, Klymko K, Bartesaghi A, Borgnia MJ, Mayer ML, and Subramaniam S. (2013) Glutamate receptor desensitization is mediated by changes in quaternary structure of the ligand binding domain.
Proc Natl Acad Sci U S A. 2013 Apr 9;110(15):5921-6. doi: 10.1073/pnas.1217549110. Epub 2013 Mar 25.

Harris AK, Meyerson JR, Matsuoka Y, Kuybeda O, Moran A, Bliss D, Das SR, Yewdell JW, Sapiro G, Subbarao K, and Subramaniam S. (2013) Structure and accessibility of HA trimers on intact 2009 H1N1 pandemic influenza virus to stem region-specific neutralizing antibodies.
Proc Natl Acad Sci U S A. 2013 Mar 19;110(12):4592-7. doi: 10.1073/pnas.1214913110. Epub 2013 Mar 4.

Milne JLS, Borgnia MJ, Bartesaghi A, Tran EEH, Earl LA, Schauder DM, Lengyel J, Pierson J, Patwardhan A, and Subramaniam S. (2013) Cryo-electron microscopy: A primer for the non-microscopist.
FEBS J. 2013 Jan;280(1):28-45. doi: 10.1111/febs.12078.

Meyerson JR, Tran EEH, Kuybeda O, Chen W, Dimitrov DS, Gorlani A, Verrips T, Lifson JD, and Subramaniam S. (2013) Molecular structures of trimeric HIV-1 Env in complex with small antibody derivatives.
Proc Natl Acad Sci U S A. 2013 Jan 8;110(2):513-8. doi: 10.1073/pnas.1214810110.

Milne, JLS. (2013) Structure and Regulation of Pyruvate Dehydrogenases.
The Encyclopedia of Biological Chemistry. Lennarz WJ and Lane MD, eds. vol. 4, pp. 321-328. Waltham, MA: Academic Press.

Karnik S and Subramaniam S. (2012) There is no overkill in biochemistry.
Resonance J Sci Edu. 2012 Dec;17(12)1157-64. doi: 10.1007/s12045-012-0132-6.

Patwardhan A, Carazo JM, Carragher B, Henderson R, Heymann JB, Hill E, Jensen GJ, Lagerstedt I, Lawson CL, Ludtke SJ, Mastronarde D, Moore WJ, Roseman A, Rosenthal P, Sorzano CO, Sanz-Garcia E, Scheres SH, Subramaniam S, Westbrook J, Winn M, Swedlow JR, and Kleywegt GJ. (2012) Data management challenges in three-dimensional EM.
Nat Struct Mol Biol. 2012 Dec 5;19(12):1203-7. doi: 10.1038/nsmb.2426.

Bartesaghi A, Lecumberry F, Sapiro G, and Subramaniam S. (2012) Protein secondary structure determination by constrained single particle cryo-electron tomography.
Structure. 2012 Dec 5;20(12):2003-13. doi: 10.1016/j.str.2012.10.016.

Kuybeda O, Frank GA, Bartesaghi A, Borgnia M, Subramaniam S, and Sapiro G. (2012) A collaborative framework for 3D alignment and classification of heterogeneous subvolumes in cryo-electron tomography.
J Struct Biol. 2012 Oct 27. pii: S1047-8477(12)00292-4. doi: 10.1016/j.jsb.2012.10.010. [Epub ahead of print].

Gopinath A, Xu G, Ress D, Oktem O, Subramaniam S, Bajaj C. (2012) Shape-based regularization of electron tomographic reconstruction.
IEEE Trans Med Imaging. 2012 Aug 20; [Epub ahead of print].

Yoo TS, Bliss D, Lowekamp BC, Chen DT, Muphy GE, Narayan K, Hartnell LM, Do T, and Subramaniam S. (2012) Visualizing cells and humans in 3D: Biomedical image analysis at nanometer and meter scales.
IEEE Comput Graph. 2012 Sep; 32(5):39-49.

Tran EEH, Borgnia MJ, Kuybeda O, Shauder DM, Bartesaghi A, Frank GA, Sapiro G, Milne JLS, Subramaniam S. (2012) Structural mechanism of trimeric HIV-1 envelope glycoprotein activation.
PLoS Pathog. 2012 Jul;8(7): e1002797.

Venkatesh MJ and Subramaniam S. (2012) Chemoreceptor arrays.
Encyclopedia of Biophysics. Gordon GKC, ed. Springer (NY).
doi: 10.1007/978-3-642-16712-6_79.

Narayan K, Prosa T, Fu J, Kelly TF, and Subramaniam S. (2012) Chemical mapping of mammalian cells by atom probe tomography.
J Struct Biol. 2012 May;178(2):98-107.

Frank GA, Bartesaghi A, Kuybeda O, Borgnia MJ, White TA, Sapiro G and Subramaniam S. (2012) Computational separation of conformational heterogeneity using cryo-electron tomography and 3D sub-volume averaging.
J Struct Biol. 2012 May;178(2):165-76.

Meyerson JR, White TA, Bliss D, Moran A, Bartesaghi A, Borgnia MJ, de la Cruz MJ, Schauder D, Hartnell LM, Nandwani R, Dawood M, Kim B, Kim JH, Sununu J, Yang L, Bhatia S, Subramaniam C, Hurt DE, Gaudreault L, Subramaniam S. (2011) Determination of Molecular Structures of HIV Envelope Glycoproteins using Cryo-Electron Tomography and Automated Sub-tomogram Averaging.
J Vis Exp. 2011 Dec 1;(58). pii: 2770, doi: 10.3791/2770.

White TA, Bartesaghi A, Borgnia MJ, de la Cruz MJ, Nandwani R, Hoxi, JA, Bess JW, Lifson JD, Milne JLS and Subramaniam S. (2011) 3D structures of sCD4-bound states of trimeric SIV envelope glycoproteins determined using cryo-electron tomography.
J Virol. 2011 Dec;85(23): 12114-23.

Murphy GE, Narayan K, Lowekamp BC, Hartnell LM, Heymann JAW, Fu J and Subramaniam S. (2011) Correlative 3D imaging of Whole Mammalian Cells with Light and Electron Microscopy.
J Struct Biol. 2011 Dec;176(3):268-78.

Harris A, Borgnia MJ, Shi D, Bartesaghi A, He H, Pejchal R, Kang K, Depetris R, Marozsan A, Sanders RW, Klasse PJ, Milne JLS, Wilson IA, Olson W, Moore JP and Subramaniam S. (2011) Trimeric HIV-1 gp140 immunogens and native HIV-1 envelope glycoproteins display the same closed and open quaternary molecular architectures.
Proc Natl Acad Sci U S A. 2011 Jul 12;108(28):11440-5.

Nikolic DS, Lehmann M, Felts RL, Garcia E, Blanchet FB, Subramaniam S and Piguet V. (2011) HIV-1 activates Cdc42 and induces filopodia in immature dendritic cells to facilitate cell-to-cell virus propagation.
Blood. 2011 Nov 3;118(18):4841-52.

Tarasov SG, Gaponenko V, Howard Z, Chen Y, Oppenheim JJ, Dyba MA, Subramaniam S, Leeb Y, Michejdaa CJ, Tarasova NI. (2011) Structural plasticity of a transmembrane peptide allows self-assembly into biologically active nanoparticles.
Proc Natl Acad Sci U S A. 2011 Jun 14;108(24):9798-803.

Khursigara C, Lan G, Neumann S, Wu X, Ravindran S, Borgnia M J, Sourjik V, Milne JLS, Tu Y and Subramaniam S. (2011) Lateral density of receptor arrays in the membrane plane influences sensitivity of the E.coli chemotaxis response.
EMBO J. 2011 May 4;30(9):1719-29.

Szakal C, Narayan K, Fu J, Lefman J and Subramaniam S. (2011) Compositional Mapping of the surface and interior of mammalian cells at submicrometer resolution.
Anal Chem. 2011 Feb 15;83(4):1207-13.

Li M, Khursigara CM, Subramaniam S and Hazelbauer G. (2011) Chemotaxis Kinase CheA is activated by three neighboring chemoreceptor dimers as effectively as by receptor clusters.
Mol Microbiol. 2011 Feb;79(3):677-85.

Butan C, Hartnell LM, Fenton AK, Bliss D, Sockett R E, Subramaniam S and Milne JLS. (2011) Spiral architecture of the nucleoid in Bdellovibrio bacteriovorus.
J Bacteriol. 2011 Mar;193(6):1341-50.

Fenton A, Hobley L, Butan C, Subramaniam S and Sockett RE. (2010) A Coiled-Coiled-Repeat-Protein ‘CCRP’ in Bdellovibrio bacteriovorus prevents cellular indentation but is not essential for vibroid cell morphology.
FEMS Microbiol Lett. 2010 Dec;313(2):89-95.

White TA, Bartesaghi A, Borgnia MJ, Meyerson JR, de la Cruz M JV, Bess JW, Lifson JD, Hoxie JA, Nandwani R, Milne JLS. and Subramaniam, S. (2010) Molecular architectures of trimeric SIV and HIV-1 envelope glycoproteins on intact viruses: Strain-dependent variation in quaternary structure.
PLoS Pathog. 2010 Dec 23;6(12):e1001249.

Felts RL, Narayan K, Estes JD, Shi D, Trubey CM, Fu J, Hartnell L, Ruthe GT, Schneider DK, Nagashima K, Bess, Jr. JW, Bavari S, Lowekamp BC, Bliss D, Lifson JD and Subramaniam S. (2010) 3D visualization of HIV transfer at the virological synapse between dendritic cells and T- cells.
Proc Natl Acad Sci U S A. 2010 Jul 27;107(30):13336-41.

Murphy GE, Lowekamp BC, Zerfas PM, Chandler RJ, Narasimha R, Venditti CP, Subramaniam S. (2010) Ion-abrasion scanning electron microscopy reveals distorted liver mitochondrial morphology in murine methylmalonic acidemia.
J Struct Biol. 2010 Aug;171(2):125-32.

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